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anti mouse il 17 mab  (Bio X Cell)


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    Structured Review

    Bio X Cell anti mouse il 17 mab
    Anti Mouse Il 17 Mab, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+il+17+mab/InVivoMAb+anti-mouse+IL-17A/pmc10856865-55-20-23
    Average 96 stars, based on 183 article reviews
    anti mouse il 17 mab - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Enzyme-linked Immunospot:

    Article Title: Serum Neuroinflammatory Disease-Induced Central Nervous System Proteins Predict Clinical Onset of Experimental Autoimmune Encephalomyelitis
    Article Snippet: .. In brief, ELISPOT plates (Millipore, Billerica, MA, USA) were precoated with anti-mouse-IFN-γ mAb (eBioscience, AN-18) and anti-mouse-IL-17 mAb (Bio X Cell; 17F3). .. Splenocytes (5 × 10 5 cells/well) and brain-infiltrating mononuclear cells (2–5 × 10 4 cells/well) were restimulated with MOG 35–55 peptide in HL-1 medium (Lonza) at 37°C for 24 h. Biotinylated anti-mouse-IFN-γ mAb (eBioscience; R4-6A2) and anti-mouse-IL-17 mAb (BioLegend, TC11-8H4) were then added overnight at 4°C, followed by incubation with streptavidin alkaline phosphatase (Invitrogen, Waltham, MA, USA) for 2 h at room temperature and developing with BCIP/NBT Phosphatase Substrate (KPL, Gaithersburg, MD, USA).

    Article Title: Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis
    Article Snippet: .. ELISPOT plates (Millipore) were coated with functional-grade purified anti-mouse IFN-γ mAb (eBioscience; AN-18) and anti-mouse IL-17 mAb (Bio X Cell; 17F3) at 4°C overnight. .. Mouse splenocytes (1×10 6 cells per well) and brain infiltrating mononuclear cells (isolated by Lympholyte-M media, Cedarlane; 2-5 ×10 4 cells per well) were collected and restimulated with MOG35-55 peptide or PV-267 as control on ELISPOT plates in HL-1 medium (Lonza BioWhittaker, Fisher Scientific) at 37°C for 24 h. Biotinylated anti-mouse IFN-γ mAb (eBioscience; R4-6A2) and anti-mouse IL-17 mAb (BioLegend; TC11-8H4) were then added to the plate overnight at 4°C followed by incubation with streptavidin alkaline phosphatase (Invitrogen) for 2 h at RT and developing with BCIP/NBT Phosphatase substrate (KPL).

    Article Title: Dietary Supplementation with 23-Hydroxy Ursolic Acid Reduces the Severity and Incidence of Acute Experimental Autoimmune Encephalomyelitis (EAE) in a Murine Model of Multiple Sclerosis
    Article Snippet: .. In brief, ELISPOT plates (Millipore, Billerica, MA, USA) were precoated with anti-mouse-IFN-γ mAb (eBioscience, San Diego, CA, USA, AN-18) and anti-mouse-IL-17 mAb (17F3, Bio X Cell, Lebanon, NH, USA). .. Splenocytes (5 × 10 5 cells/well) were restimulated with MOG35–55 peptide in HL-1 medium (Lonza, Basel, Switzaerland) at 37 °C, 5% CO 2 for 24 h. Biotinylated anti-mouse-IFN-γ mAb (R4-6A2, eBioscience/Thermo Fisher, Waltham, MA, USA) and anti-mouse-IL-17 mAb (TC11-8H4, BioLegend, San Diego, CA, USA) were then added overnight at 4 °C, followed by incubation with streptavidin alkaline phosphatase (Invitrogen, Waltham, MA, USA) for 2 h at room temperature and developing with BCIP/NBT Phosphatase Substrate (KPL, Gaithersburg, MD, USA).

    Article Title: Macrophage migration inhibitory factor promotes resistance to glucocorticoid treatment in EAE
    Article Snippet: .. In brief, ELISPOT plates (Millipore, Billerica, MA) were precoated with anti-mouse-IFN-γ mAb (eBioscience, San Diego, CA; AN-18) and anti-mouse-IL-17 mAb (Bio X Cell; 17F3). .. Splenocytes (1 × 10 6 cells per well) and brain-infiltrating mononuclear cells (MNCs; 2–5 × 10 4 cells per well) were restimulated with MOG35-55 peptide in HL-1 medium (Lonza, Basel, Switzerland) at 37°C for 24 hours.

    Article Title: Macrophage migration inhibitory factor promotes resistance to glucocorticoid treatment in EAE
    Article Snippet: .. Cytokine ELISPOT assay was performed and spots analyzed as described previously.20 In brief, ELISPOT plates (Millipore, Billerica, MA) were precoated with anti-mouse-IFN-g mAb (eBioscience, San Diego, CA; AN-18) and anti-mouse-IL-17 mAb (Bio X Cell; 17F3). .. Splenocytes (13 106 cells per well) and brain-infiltrating mononuclear cells (MNCs; 2–5 3 104 cells per well) were restimulated with MOG35-55 peptide in HL-1 medium (Lonza, Basel, Switzerland) at 37°C for 24 hours.

    Article Title: TNFR2 limits proinflammatory astrocyte functions during EAE induced by pathogenic DR2b-restricted T cells
    Article Snippet: .. In brief, ELISPOT plates (MilliporeSigma) were precoated with anti-mouse IFN-γ mAb (eBioscience, AN-18) and anti-mouse IL-17 mAb (Bio X Cell, 17F3). .. Splenocytes (5 × 10 5 cells per well) and brain-infiltrating mononuclear cells (2 × 10 4 to 5 × 10 4 cells per well) were restimulated with MOG 35-55 peptide in HL-1 medium (Lonza) at 37°C for 24 hours.

    Control:

    Article Title: Targeting resident memory T cell immunity culminates in pulmonary and systemic protection against Brucella infection
    Article Snippet: .. Half of each group received IP 250 μg of either of rat IgG1 isotype control (clone TNP6A7, BioXCell) or anti-mouse IL-17 mAb (Clone 17F3, BioXCell) on days -1, day 2, 7, and 12 post-challenge. ..

    Functional Assay:

    Article Title: Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis
    Article Snippet: .. ELISPOT plates (Millipore) were coated with functional-grade purified anti-mouse IFN-γ mAb (eBioscience; AN-18) and anti-mouse IL-17 mAb (Bio X Cell; 17F3) at 4°C overnight. .. Mouse splenocytes (1×10 6 cells per well) and brain infiltrating mononuclear cells (isolated by Lympholyte-M media, Cedarlane; 2-5 ×10 4 cells per well) were collected and restimulated with MOG35-55 peptide or PV-267 as control on ELISPOT plates in HL-1 medium (Lonza BioWhittaker, Fisher Scientific) at 37°C for 24 h. Biotinylated anti-mouse IFN-γ mAb (eBioscience; R4-6A2) and anti-mouse IL-17 mAb (BioLegend; TC11-8H4) were then added to the plate overnight at 4°C followed by incubation with streptavidin alkaline phosphatase (Invitrogen) for 2 h at RT and developing with BCIP/NBT Phosphatase substrate (KPL).

    Purification:

    Article Title: Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis
    Article Snippet: .. ELISPOT plates (Millipore) were coated with functional-grade purified anti-mouse IFN-γ mAb (eBioscience; AN-18) and anti-mouse IL-17 mAb (Bio X Cell; 17F3) at 4°C overnight. .. Mouse splenocytes (1×10 6 cells per well) and brain infiltrating mononuclear cells (isolated by Lympholyte-M media, Cedarlane; 2-5 ×10 4 cells per well) were collected and restimulated with MOG35-55 peptide or PV-267 as control on ELISPOT plates in HL-1 medium (Lonza BioWhittaker, Fisher Scientific) at 37°C for 24 h. Biotinylated anti-mouse IFN-γ mAb (eBioscience; R4-6A2) and anti-mouse IL-17 mAb (BioLegend; TC11-8H4) were then added to the plate overnight at 4°C followed by incubation with streptavidin alkaline phosphatase (Invitrogen) for 2 h at RT and developing with BCIP/NBT Phosphatase substrate (KPL).



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